Abstract: This study used ion transport and chemical reaction in biological nanopore and nanochannel as the background, with ion current rectification and ion accumulation and depletion model as the model basis. The ion distribution and velocity field in nanochannel under pressure and electric field interaction were studied by using finite element method. By changing the magnitude of the electric field, the effect of the interaction between the pressure and the electric field on the velocity field, flow field and ion distribution in the conical nanopore was observed. Results show that hydrogen ion movement direction is mainly influenced by electric field direction. Due to electrostatic adsorption effect, hydrogen ion concentration near the layer is higher than the bulk hydrogen ion concentration. When the voltage is low, the direction of the flow field is mainly affected by the pressure flow. When the voltage is high, the flow direction of the flow field is determined by the electroosmotic flow and pressure-driven flow.