Abstract: A vertical fence with a height h was mounted inside the zero-pressure-gradient turbulent boundary layer, and the streamwise evolution of the properties of the turbulent/non-turbulent interface(TNTI) was studied under the influence of spanwise vortices induced by the fence. It is shown that the flow experiences a process from turbulent boundary layer(TBL) to flow separation and reattachment and finally recovering to TBL. The influence of the fence is weakened after a distance of about 18h downstream of the fence and the wall shear contribution to turbulence intensity recovers gradually, and TBL is fully developed again after a distance of about 55h downstream of the fence under current experimental conditions. Affected by the fence, the fractal dimension of the TNTI is decreased by the enhancement of coherence of the structures in the flow field, which indicates that the shedding vortices weaken the multi-scale characteristics and three-dimensionality of the TNTI. In addition, the probability density function of the interface heights shows a significant positive skewness, which is mainly related to the rising of the TNTI induced by the enhanced ejections. The characteristics of flow structures and TNTI change synchronously in the same streamwise region. The influence of the fence on these characteristics of the TNTI is mainly concentrated in the streamwise region of (-5~18)h.